-
Product Name
JAM3 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to JAM3
-
Tested applications
WB
-
Species reactivity
Human
-
Alternative names
JAM3 antibody; JAM-2 antibody; JAM-3 antibody; JAM-C antibody; JAMC antibody; junctional adhesion molecule C antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human JAM3 (NP_116190.3).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
-
Validations
Western blot - JAM3 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using JAM3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.
-
Background
Mediates cell-cell adhesion. Functions as counter-receptor for JAM2. Functions as a counter-receptor for ITGAM, mediating leukocyte-platelet interactions and is involved in the regulation of transepithelial migration of polymorphonuclear neutrophils (PMN). Plays a role in angiogenesis. May play a role in the regulation of cell migration (Probable). Required for normal polarization and acrosome formation in developing spermatids, and for normal male fertility (By similarity).; Soluble form of JAM-C: Promotes chemotaxis of vascular endothelial cells and stimulates angiogenesis.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"