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Product Name
JAKMIP1 antibody
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Description
JAKMIP1 Rabbit Polyclonal antibody. Positive IP detected in Jurkat cells. Positive WB detected in mouse spleen tissue, HEK-293 cells, Jurkat cells, mouse liver tissue, mouse testis tissue, Y79 cells. Positive FC detected in Jurkat cells. Positive IHC detected in human gliomas tissue. Observed molecular weight by Western-blot: 73 kDa
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Tested applications
ELISA, WB, IHC, IP, FC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
FLJ31564 antibody; Gababrbp antibody; JAKMIP1 antibody; JAMIP1 antibody; Marlin 1 antibody; MARLIN1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of JAKMIP1 recombinant protein (Accession Number: NM_144720). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
mouse spleen tissue were subjected to SDS PAGE followed by western blot with Catalog No:111880(JAKMIP1 antibody) at dilution of 1:800
IP Result of anti-JAKMIP1 (IP:Catalog No:111880, 3ug; Detection:Catalog No:111880 1:1000) with Jurkat cells lysate 4000ug.
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:111880(JAKMIP1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:111880(JAKMIP1 antibody) at dilution of 1:100 (under 40x lens)
1X10^6 Jurkat cells were stained with 0.2ug JAKMIP1 antibody (Catalog No:111880, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
JAKMIP1 represents janus kinase and microtubule interacting protein 1
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References
- Okai I, Wang L, Gong L. Overexpression of JAKMIP1 associates with Wnt/β-catenin pathway activation and promotes cancer cell proliferation in vitro. Biomedicine & pharmacotherapy = Biomédecine & pharmacothérapie. 67(3):228-34. 2013.
- Berg JM, Lee C, Chen L. JAKMIP1, a Novel Regulator of Neuronal Translation, Modulates Synaptic Function and Autistic-like Behaviors in Mouse. Neuron. 88(6):1173-91. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"