IYD Polyclonal Antibody

Cat.#: 166071

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Product Information

  • Product Name
    IYD Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to IYD
  • Tested applications
    WB
  • Species reactivity
    Mouse
  • Alternative names
    IYD antibody; C6orf71 antibody; DEHAL1 antibody; IYD-1 antibody; TDH4 antibody; iodotyrosine deiodinase antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 24-214 of human IYD (NP_981932.1).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:200 - 1:2000
  • Validations

    Western blot - IYD Polyclonal Antibody

    Western blot - IYD Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using IYD antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.

  • Background
    Catalyzes the oxidative NADPH-dependent deiodination of monoiodotyrosine (L-MIT) or diiodotyrosine (L-DIT). Acts during the hydrolysis of thyroglobulin to liberate iodide, which can then reenter the hormone-producing pathways. Acts more efficiently on monoiodotyrosine than on diiodotyrosine.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"