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Product Name
ITPR1-specific antibody
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Description
ITPR1-specific Rabbit Polyclonal antibody. Positive WB detected in mouse brain tissue, mouse liver tissue. Positive FC detected in HepG2 cells. Positive IHC detected in human testis tissue. Observed molecular weight by Western-blot: 290-300 kDa
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Tested applications
ELISA, WB, IHC, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
INSP3R1 antibody; IP3 receptor isoform 1 antibody; IP3R antibody; IP3R 1 antibody; IP3R1 antibody; ITPR1 antibody; SCA15 antibody; SCA16 antibody; Type 1 InsP3 receptor antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_001099952). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:111960(ITPR1-specific antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human testis using Catalog No:111960(ITPR1-specific antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:111960(ITPR1-specific antibody) at dilution of 1:100 (under 40x lens)
1X10^6 HepG2 cells were stained with .2ug ITPR1-specific antibody (Catalog No:111960, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
ITPR1, also named as INSP3R1 and IP3R, belongs to the InsP3 receptor family. It is a receptor for inositol 1,4,5-trisphosphate which is a second messenger that mediates the release of intracellular calcium. Defects in ITPR1 are the cause of spinocerebellar ataxia type 15 (SCA15). The antibody has no cross reaction with ITPR2 and ITPR3.
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