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Product Name
ITPKA antibody
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Description
ITPKA Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in mouse brain tissue. Positive IF detected in HepG2 cells. Positive IHC detected in human gliomas tissue. Observed molecular weight by Western-blot: 51 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
InsP 3 kinase A antibody; IP3 3 kinase A antibody; IP3K A antibody; IP3KA antibody; ITPKA antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ITPKA recombinant protein (Accession Number: NM_002220). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:111957(ITPKA antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:111957(ITPKA antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:111957(ITPKA antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using ITPKA antibody Catalog No:111957 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-ITPKA (IP:Catalog No:111957, 4ug; Detection:Catalog No:111957 1:400) with mouse brain tissue lysate 4000ug.
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Background
ITPKA(Inositol-trisphosphate 3-kinase A) belongs to the inositol phosphokinase (IPK) family. It accumulates in dendritic spines and seems to be critically involved in synaptic plasticity. The protein possesses two functional activities. In neurons, ITPKA is targeted to F-actin via an N-terminal actin binding domain (amino acids 1–66) and is relevant for long term potentiation and spatial learning(PMID:20022963).
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References
- Windhorst S, Kalinina T, Schmid K. Functional role of inositol-1,4,5-trisphosphate-3-kinase-A for motility of malignant transformed cells. International journal of cancer. 129(6):1300-9. 2011.
- Ashour DJ, Pelka B, Jaaks P. The catalytic domain of inositol-1,4,5-trisphosphate 3-kinase-a contributes to ITPKA-induced modulation of F-actin. Cytoskeleton (Hoboken, N.J.). 72(2):93-100. 2015.
- Li J, Zhu YH, Huang P, Zhang B, Sun J, Guan XY. ITPKA expression is a novel prognostic factor in hepatocellular carcinoma. Diagnostic pathology. 10:136. 2015.
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