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Product Name
ITGB8 Polyclonal Antibody
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Description
Polyclonal antibody to ITGB8
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
ITGB8 antibody; integrin beta-8 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 43-320 of human ITGB8 (NP_002205.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - ITGB8 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ITGB8 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.
Immunohistochemistry - ITGB8 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using ITGB8 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - ITGB8 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using ITGB8 antibody at dilution of 1:100 (40x lens).
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Background
Integrin alpha-V:beta-8 (ITGAV:ITGB8) is a receptor for fibronectin. It recognizes the sequence R-G-D in its ligands. Integrin alpha-V:beta-6 (ITGAV:ITGB6) mediates R-G-D-dependent release of transforming growth factor beta-1 (TGF-beta-1) from regulatory Latency-associated peptide (LAP), thereby playing a key role in TGF-beta-1 activation on the surface of activated regulatory T-cells (Tregs) (Probable). Required during vasculogenesis (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"