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Product Name
IRF2 Polyclonal Antibody
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Description
Polyclonal antibody to IRF2
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
IRF2 antibody; IRF-2 antibody; interferon regulatory factor 2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 120-349 of human IRF2 (NP_002190.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100 -
Validations
Western blot - IRF2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using IRF2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry - IRF2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human breast cancer using IRF2 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - IRF2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat heart using IRF2 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - IRF2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using IRF2 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - IRF2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human stomach cancer using IRF2 antibody at dilution of 1:200 (40x lens).
Immunofluorescence - IRF2 Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using IRF2 antibody . Blue: DAPI for nuclear staining.
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Background
Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) and represses those genes. Also acts as an activator for several genes including H4 and IL7. Constitutively binds to the ISRE promoter to activate IL7. Involved in cell cycle regulation through binding the site II (HiNF-M) promoter region of H4 and activating transcription during cell growth. Antagonizes IRF1 transcriptional activation.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"