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Product Name
IQSEC1 Polyclonal Antibody
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Description
Polyclonal antibody to IQSEC1
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Tested applications
WB
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Species reactivity
Mouse, Rat
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Alternative names
IQSEC1 antibody; ARF-GEP100 antibody; ARFGEP100 antibody; BRAG2 antibody; GEP100 antibody; IQ motif and Sec7 domain 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 180-380 of human IQSEC1 (NP_055684.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
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Validations
Western blot - IQSEC1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using IQSEC1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 5min.
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Background
Guanine nucleotide exchange factor for ARF1 and ARF6. Guanine nucleotide exchange factor activity is enhanced by lipid binding. Accelerates GTP binding by ARFs of all three classes. Guanine nucleotide exchange protein for ARF6, mediating internalisation of beta-1 integrin.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"