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Product Name
IQGAP1 antibody
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Description
IQGAP1 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, A549 cells, HEK-293 cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, SGC-7901 cells. Positive IF detected in HeLa cells. Positive IHC detected in human kidney tissue, human liver cancer tissue. Observed molecular weight by Western-blot: 190-195 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
HUMORFA01 antibody; IQGAP1 antibody; KIAA0051 antibody; p195 antibody; SAR1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of IQGAP1 recombinant protein (Accession Number: NM_003870). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:111824(IQGAP1 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human kidney slide using Catalog No:111824(IQGAP1 Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human kidney slide using Catalog No:111824(IQGAP1 Antibody) at dilution of 1:50
Immunofluorescent analysis of HeLa cells using Catalog No:111824(IQGAP1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
IQGAP1 represents IQ motif containing GTPase activating protein 1
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References
- Moon H, Ruelcke JE, Choi E. Diet-induced hypercholesterolemia promotes androgen-independent prostate cancer metastasis via IQGAP1 and caveolin-1. Oncotarget. 6(10):7438-53. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"