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Product Name
INTS6 Polyclonal Antibody
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Description
Polyclonal antibody to INTS6
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
INTS6 antibody; DBI-1 antibody; DDX26 antibody; DDX26A antibody; DICE1 antibody; HDB antibody; INT6 antibody; Notchl2 antibody; integrator complex subunit 6 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human INTS6 (NP_036273.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - INTS6 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using INTS6 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - INTS6 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human breast cancer using INTS6 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - INTS6 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse lung using INTS6 Antibody at dilution of 1:100 (40x lens).
Immunofluorescence - INTS6 Polyclonal Antibody
Immunofluorescence analysis of A549 cells using INTS6 antibody . Blue: DAPI for nuclear staining.
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Background
Component of the Integrator (INT) complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes (Probable). Mediates recruitment of cytoplasmic dynein to the nuclear envelope, probably as component of the INT complex. May have a tumor suppressor role; an ectopic expression suppressing tumor cell growth.
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