-
Product Name
Mouse IL-5/IL5 / Interleukin 5 (His Tag) recombinant protein
- Documents
-
Description
Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.
-
Protein name
Interleukin-5
-
Protein short names
TRF; EDF; RP23-239O19.2; IL-5
-
Uniprot ID
P04401
-
Gene Name
Il5; Il-5
-
Source/Expression Host
Baculovirus-Insect Cells
-
Expression Plasmid/cDNA
A DNA sequence encoding the mouse IL5 (P04401) (Met 1-Gly 133) was expressed?with a C-terminal polyhistidine tag.
-
Protein Species
Mouse
-
Molecular weight
The recombinant mouse IL5 consists of 123 amino acids and has a calculated molecular mass of 14.5 kDa. It migrates as an approximately 18 kDa band in SDS-PAGE under reducing conditions, and the homodimer form could also be seen.
-
Purity
> 90 % as determined by SDS-PAGE
-
Activity
1. Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse IL5-his at 5 μg/ml (100 μl/well) can bind biotinylated human IL5Ra-his with a linear range of 32-4000 ng/mL.
2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.1-0.5 ng/mL. -
Validations
Mouse IL5 Protein (His Tag) SDS-PAGE
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"