Mouse IL18RAP (His Tag) recombinant protein

Mouse IL18RAP (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Interleukin 18 receptor accessory protein, also known as IL18RAP and CDw218b (cluster of differentiation w218b), is an accessory subunit of the heterodimeric receptor for IL18. This protein enhances the IL18 binding activity of IL18R1 (IL1RRP), a ligand binding subunit of IL18 receptor. The coexpression of IL18R1 and this protein is required for the activation of NF-kappaB and MAPK8 (JNK) in response to IL18. IL18RAP is required for the high affinity binding of interleukin 18 (IL-18) to its receptor complex. IL18RAP together with IL18R1 mediates IL-18-dependent activation of NF-kappa-B and JNK. Two putative isoforms of IL18RAP were detected and the ratios and total levels of these isoforms may contribute to the aetiology of coeliac disease. IL18R1 and IL18RAP polymorphisms have been found associated with diseases such as schizophrenia, HSV1 seropositivity and atopic asthma. Analysis of IL18R1 and IL18RAP SNPs in 5 European prospective cohorts suggests that the variability of these genes are unlikely to contribute to modulate the risk of CVD in European populations.

Interleukin 18 receptor accessory protein

Q9Z2B1

Il18rap; mCG_4977

Baculovirus-Insect Cells

A DNA sequence encoding the mouse IL18RAP (NP_034683.1) (Met1-Glu356) was expressed with a C-terminal polyhistidine tag.

Mouse

The recombinant mouse IL18RAP comprises 348 amino acids and has a predicted molecular mass of 40.1 kDa. The apparent molecular mass of the protein is approximately 44 kDa in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA.
Immobilized mouse IL18RAP-His at 10 μg/ml (100 μl/well) can bind biotinylated mouse IL18 (Cat:501172), The EC₅₀ of biotinylated mouse IL18 (Cat:501172) is 10.6-25.0 μg/ml.