Product Name

Integrin alpha-4 antibody

Documents

Description

Integrin alpha-4 Rabbit Polyclonal antibody. Positive IHC detected in human tonsillitis tissue. Positive WB detected in Jurkat cells. Observed molecular weight by Western-blot: 115 kDa

Tested applications

ELISA, WB, IHC

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

CD49D antibody; IA4 antibody; Integrin alpha 4 antibody; Integrin alpha IV antibody; ITGA4 antibody; VLA 4 subunit alpha antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of Peptide (Accession Number: NM_000885). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:1000-1:10000

IHC: 1:20-1:200

Validations

Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:111859(ITGA4 antibody) at dilution of 1:3000

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:111859(Integrin alpha-4 Antibody) at dilution of 1:50

Background

ITGA4, also named as CD49D, belongs to the integrin alpha chain family. ITGA4 is a receptor for fibronectin, VCAM1 and MADCAM1. ITGA4 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. It recognizes the sequence Q-I-D-S in VCAM1. I It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells ITGA4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.

References

Zhao XP, Wang M, Song Y. Membrane microvesicles as mediators for melanoma-fibroblasts communication: roles of the VCAM-1/VLA-4 axis and the ERK1/2 signal pathway. Cancer letters. 360(2):125-33. 2015.

Integrin alpha-4 antibody

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