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Product Name
Integrin alpha-3 antibody
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Description
Integrin alpha-3 Rabbit Polyclonal antibody. Positive IF detected in A549 cells. Positive FC detected in PC-3 cells. Positive WB detected in PC-3 cells, A549 cells, human brain tissue, human heart tissue, human testis tissue. Positive IP detected in PC-3 cells. Observed molecular weight by Western-blot: 120 kDa
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Tested applications
ELISA, WB, IF, IP, FC
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Species reactivity
Human; other species not tested.
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Alternative names
CD49C antibody; FLJ34631 antibody; FLJ34704 antibody; FRP 2 antibody; Galactoprotein B3 antibody; GAP B3 antibody; GAPB3 antibody; Integrin alpha 3 antibody; ITGA3 antibody; MSK18 antibody; VCA 2 antibody; VL3A antibody; VLA 3 subunit alpha antibody; VLA3a antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Integrin alpha-3 recombinant protein (Accession Number: NM_002204). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IF: 1:20-1:200
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Validations
PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:111858(ITGA3 antibody) at dilution of 1:500
IP Result of anti-Integrin alpha-3 (IP:Catalog No:111858, 4ug; Detection:Catalog No:111858 1:500) with PC-3 cells lysate 1200ug.
Immunofluorescent analysis of A549 cells using Catalog No:111858(Integrin alpha-3 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
1X10^6 PC-3 cells were stained with 0.2ug Integrin alpha-3 antibody (Catalog No:111858, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
ITGA3, also named as MSK18, CD49c, GAPB3 and FRP-2, belongs to the integrin alpha chain family. It is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. ITGA3 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. ITGA3 is highly concentrated in epithelial cells where it strongly adheres to Laminin-5 and Laminin-5 induced rapid adhesion can be blocked by antibodies against the alpha-3 integrin subunit. It can be cleaved into heavy chain(840aa) and light chain(176aa). This antibody can recognize the heavy chain.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"