Human IGF1R/CD221 (His & GST Tag) recombinant protein

Human IGF1R/CD221 (His & GST Tag) recombinant protein

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The insulin-like growth factor-1 receptor (IGF1R) is a transmembrane tyrosine kinase involved in several biological processes including cell proliferation, differentiation, DNA repair, and cell survival. This a disulfide-linked heterotetrameric transmembrane protein consisting of two α and two β subunits, and among which, the α subunit is extracellular while the β subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. IGF1R signalling pathway is activated in the mammalian nervous system from early developmental stages. Its major effect on developing neural cells is to promote their growth and survival. This pathway can integrate its action with signalling pathways of growth and morphogenetic factors that induce cell fate specification and selective expansion of specified neural cell subsets. Modulation of cell migration is another possible role that IGF1R activation may play in neurogenesis. In the mature brain, IGF-I binding sites have been found in different regions of the brain, and multiple reports confirmed a strong neuroprotective action of the IGF-IR against different pro-apoptotic insults. IGF1R is an important signaling molecule in cancer cells and plays an essential role in the establishment and maintenance of the transformed phenotype. Inhibition of IGF1R signaling thus appears to be a promising strategy to interfere with the growth and survival of cancer cells. IGF1R is frequently overexpressed by tumours, and mediates proliferation and apoptosis protection. IGF signalling also influences hypoxia signalling, protease secretion, tumour cell motility and adhesion, and thus can affect the propensity for invasion and metastasis. Therefore, the IGF1R is now an attractive anti-cancer treatment target.

Insulin-like growth factor 1 receptor

D930020L01; MGC142170; MGC18216; A330103N21RIK; IGFR; HYFT; CD221; MGC142172; JTK13; IGF-1R; IGFIR; IGF1R

P08069

IGF1R

Baculovirus-Insect Cells

A DNA sequence encoding the human IGF1R (NP_000866.1) cytoplasmic domain (Met 954-Cys 1367) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Human

The recombinant human IGF1R (aa 954-1367) /GST chimera consists of 651 amino acids and has a calculated molecular mass of 74.6 KDa.

> 85 % as determined by SDS-PAGE

The specific activity was determined to be 146 nmol/min/mg using Poly(Glu,Tyr) 4:1 as substrate.