Product Name

Insulin antibody

Documents

Description

Insulin Rabbit Polyclonal antibody. Positive WB detected in BxPC-3 cells, mouse pancreas tissue. Positive IHC detected in human pancreas tissue, mouse pancreas tissue, rat pancreas tissue. Observed molecular weight by Western-blot: 30 kDa

Tested applications

ELISA, WB, IHC

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

ILPR antibody; INS antibody; insulin antibody; IRDN antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of Insulin recombinant protein (Accession Number: NM_001185097). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IHC: 1:20-1:200

Validations

BxPC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:111853(INS antibody) at dilution of 1:10000

Immunohistochemical of paraffin-embedded human pancreas using Catalog No:111853(INS antibody) at dilution of 1:500 (under 10x lens)

Immunohistochemical of paraffin-embedded human pancreas using Catalog No:111853(INS antibody) at dilution of 1:500 (under 40x lens)

Background

Insulin is a peptide hormone, produced by beta cells of the pancreas, and is central to regulating carbohydrate and fat metabolism in the body. It participates in glucose utilization, protein synthesis and in the formation and storage of neutral lipids. Insulin is synthesized as a precursor molecule, proinsulin, which is processed prior to secretion. A- and B-peptides are joined together by a disulfide bond to form insulin, while the central portion of the precursor molecule is cleaved and released as the C-peptide. Defects in insulin results in type 1 diabetes mellitus. Insulin may also exist 36 kDa form corresponding to the hexameric insulin form.

References

Feng F, Hu P, Chen L. Display of human proinsulin on the Bacillus subtilis spore surface for oral administration. Current microbiology. 67(1):1-8. 2013.
Lehner C, Gehwolf R, Wagner A. Tendons from non-diabetic humans and rats harbor a population of insulin-producing, pancreatic beta cell-like cells. Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et métabolisme. 44(7):506-10. 2012.
Maneva-Radicheva L, Amatya C, Parker C. Autoimmune diabetes is suppressed by treatment with recombinant human tissue Kallikrein-1. PloS one. 9(9):e107213. 2014.
Zheng H, Fu J, Xue P. CNC-bZIP protein Nrf1-dependent regulation of glucose-stimulated insulin secretion. Antioxidants & redox signaling. 22(10):819-31. 2015.
Fang F, Chen D, Yu P. Effects of Bisphenol A on glucose homeostasis and brain insulin signaling pathways in male mice. General and comparative endocrinology. 212:44-50. 2015.
Fang F, Gao Y, Wang T. Insulin signaling disruption in male mice due to perinatal bisphenol A exposure: Role of insulin signaling in the brain. Toxicology letters. 245:59-67. 2016.

Insulin antibody

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