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Product Name
INPP5J Polyclonal Antibody
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Description
Polyclonal antibody to INPP5J
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
INPP5J antibody; INPP5 antibody; PIB5PA antibody; PIPP antibody; inositol polyphosphate-5-phosphatase J antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 60-360 of human INPP5J (NP_001002837.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:10 - 1:100 -
Validations
Western blot - INPP5J Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using INPP5J antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - INPP5J Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat liver using INPP5J Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - INPP5J Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat kidney using INPP5J Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - INPP5J Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse heart using INPP5J Antibody at dilution of 1:100 (40x lens).
Immunofluorescence - INPP5J Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using INPP5J antibody . Blue: DAPI for nuclear staining.
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Background
Inositol 5-phosphatase, which converts inositol 1,4,5-trisphosphate to inositol 1,4-bisphosphate. Also converts phosphatidylinositol 4,5-bisphosphate to phosphatidylinositol 4-phosphate and inositol 1,3,4,5-tetrakisphosphate to inositol 1,3,4-trisphosphate in vitro. May be involved in modulation of the function of inositol and phosphatidylinositol polyphosphate-binding proteins that are present at membranes ruffles (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"