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Product Name
ING4 Polyclonal Antibody
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Description
Polyclonal antibody to ING4
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Tested applications
WB, IHC
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Species reactivity
Human
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Alternative names
ING4 antibody; my036 antibody; p29ING4 antibody; inhibitor of growth protein 4 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human ING4
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, pH7.3.
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Storage instructions
Store at 4℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
IHC 1:20 - 1:50 -
Validations
Western blot - ING4 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ING4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
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Background
Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Through chromatin acetylation it may function in DNA replication. May inhibit tumor progression by modulating the transcriptional output of signaling pathways which regulate cell proliferation. Can suppress brain tumor angiogenesis through transcriptional repression of RELA/NFKB3 target genes when complexed with RELA. May also specifically suppress loss of contact inhibition elicited by activated oncogenes such as MYC. Represses hypoxia inducible factor's (HIF) activity by interacting with HIF prolyl hydroxylase 2 (EGLN1). Can enhance apoptosis induced by serum starvation in mammary epithelial cell line HC11 (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"