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Product Name
IMP3 Polyclonal Antibody
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Description
Polyclonal antibody to IMP3
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Tested applications
WB
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Species reactivity
Human, Mouse
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Alternative names
IMP3 antibody; BRMS2 antibody; C15orf12 antibody; MRPS4 antibody; IMP3, U3 small nucleolar ribonucleoprotein antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-184 of human IMP3 (NP_060755.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:200 - 1:2000
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Validations
Western blot - IMP3 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using IMP3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.
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Background
Component of the 60-80S U3 small nucleolar ribonucleoprotein (U3 snoRNP). Required for the early cleavages during pre-18S ribosomal RNA processing.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"