ILK antibody

Cat.#: 111753

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Product Information

  • Product Name
    ILK antibody
  • Documents
  • Description
    ILK Rabbit Polyclonal antibody. Positive WB detected in mouse heart tissue, MCF-7 cells. Positive IF detected in MCF-7 cells. Observed molecular weight by Western-blot: 51 kDa
  • Tested applications
    ELISA, WB, IF
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    DKFZp686F1765 antibody; ILK antibody; ILK 1 antibody; ILK 2 antibody; ILK1 antibody; ILK2 antibody; integrin linked kinase antibody; Integrin linked protein kinase antibody; P59 antibody; p59ILK antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of ILK recombinant protein (Accession Number: NM_001014794). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IF: 1:20-1:200

  • Validations

    mouse heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:111753(ILK Antibody) at dilution of 1:1000

    mouse heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:111753(ILK Antibody) at dilution of 1:1000

    Immunofluorescent analysis of (-20oc Ethanol) fixed MCF-7 cells using Catalog No:111753(ILK Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of (-20oc Ethanol) fixed MCF-7 cells using Catalog No:111753(ILK Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    ILK(Integrin-linked protein kinase) is also named as p59, PAT-4, DKFZp686F1765, ILK-1, ILK-2, ILK1, ILK2, p59ILK and belongs to the TKL Ser/Thr protein kinase family. This protein localizes to multiple sites, including the cytoplasm, and imports into the nucleus through sequences in its N-terminus, via active transport mechanisms that involve nuclear pore complexes(PMID: 18635968). ILK performs crucial roles in the control of intestinal cell and crypt-villus axis homeostasis-especially with regard to basement membrane fibronectin deposition-as well as cell proliferation, spreading, and migration(PMID:19885839).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"