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Product Name
ILF3 antibody
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Description
ILF3 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in mouse spleen tissue, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, Raji cells. Positive IF detected in Hela cells. Positive IHC detected in human cervical cancer tissue, human placenta tissue. Observed molecular weight by Western-blot: 90kd,110kd
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Tested applications
ELISA, IHC, IF, IP, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CBTF antibody; DRBF antibody; DRBP76 antibody; ILF3 antibody; M phase phosphoprotein 4 antibody; MMP4 antibody; MPHOSPH4 antibody; MPP4 antibody; NF AT 90 antibody; NF110 antibody; NF90 antibody; NFAR antibody; NFAR 1 antibody; NFAR2 antibody; TCP110 antibody; TCP80 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ILF3 recombinant protein (Accession Number: NM_001137673). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse spleen tissue were subjected to SDS PAGE followed by western blot with Catalog No:111751(NF90,ILF3 Antibody) at dilution of 1:1000
IP Result of anti-NF90,ILF3 (IP:Catalog No:111751, 3ug; Detection:Catalog No:111751 1:1000) with HeLa cells lysate 1600ug.
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:111751(NF90,ILF3 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of Hela cells, using ILF3 antibody Catalog No:111751 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
Nuclear factor of activated T cells (NFAT)is a transcription factor required for T-cell expression of interleukin-2 (IL2). NFAT binds to a sequence known as the antigen receptor response element-2 (ARRE2) in the IL2 enhancer. NFAT contains two subunits, one is ILF3. ILF3 can facilitate double-standed RNA-regulated gene expression at the level of post-transcription. It binds to and inhibits coding sequences of acid beta-glucosidase and other mRNAs and functions at the initiation phase of GCase mRNA translation, probably by inhibiting its binding to polysomes. ILF3 exists main two isoforms NFAR-1 (90 kDa) and -2 (110 kDa). NFAR-1 and -2 each contain two DRBDs in their C-terminus regions and exhibit 98% homology to one another at the amino acid level. NFAR-2 contains an extra 192 amino acids at the C-terminal region that are encoded by an extra three exons present in its corresponding transcript.
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References
- Aras S, Pak O, Sommer N. Oxygen-dependent expression of cytochrome c oxidase subunit 4-2 gene expression is mediated by transcription factors RBPJ, CXXC5 and CHCHD2. Nucleic acids research. 41(4):2255-66. 2013.
- Aras S, Bai M, Lee I, Springett R, Hüttemann M, Grossman LI. MNRR1 (formerly CHCHD2) is a bi-organellar regulator of mitochondrial metabolism. Mitochondrion. 20:43-51. 2015.
- Agca C, Boldt K, Gubler A. Expression of leukemia inhibitory factor in Müller glia cells is regulated by a redox-dependent mRNA stability mechanism. BMC biology. 13:30. 2015.
- Zhang LF, Lou JT, Lu MH. Suppression of miR-199a maturation by HuR is crucial for hypoxia-induced glycolytic switch in hepatocellular carcinoma. The EMBO journal. 34(21):2671-85. 2015.
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