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Product Name
IL27RA Polyclonal Antibody
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Description
Polyclonal antibody to IL27RA
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
IL27RA antibody; CRL1 antibody; IL-27RA antibody; IL27R antibody; TCCR antibody; WSX1 antibody; zcytor1 antibody; interleukin-27 receptor subunit alpha antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 33-283 of human IL27RA (NP_004834.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - IL27RA Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using IL27RA antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - IL27RA Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using IL27RA antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Receptor for IL27. Requires IL6ST/gp130 to mediate signal transduction in response to IL27. This signaling system acts through STAT3 and STAT1. Involved in the regulation of Th1-type immune responses. Also appears to be involved in innate defense mechanisms.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"