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IL-6 antibody

Cat.#: 111748

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Product Information

  • Product Name
    IL-6 antibody
  • Documents
  • Description
    IL-6 Rabbit Polyclonal antibody. Positive WB detected in Recombinant protein, Transfected HEK-293 cells. Positive IHC detected in human tonsillitis tissue, human spleen tissue. Observed molecular weight by Western-blot: 30 kDa
  • Tested applications
    ELISA, WB, IHC
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    B cell stimulatory factor 2 antibody; BSF 2 antibody; BSF2 antibody; CDF antibody; CTL differentiation factor antibody; HGF antibody; HSF antibody; Hybridoma growth factor antibody; IFN beta 2 antibody; IFNB2 antibody; IL 6 antibody; IL6 antibody; IL-6 antibody; Interferon beta 2 antibody; Interleukin 6 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of IL-6 recombinant protein (Accession Number: XM_011515390). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IHC: 1:20-1:200

  • Validations

    Recombinant protein were subjected to SDS PAGE followed by western blot with Catalog No:111748(IL6 Antibody) at dilution of 1:3000

    Recombinant protein were subjected to SDS PAGE followed by western blot with Catalog No:111748(IL6 Antibody) at dilution of 1:3000

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:111748(IL6 Antibody) at dilution of 1:1000

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:111748(IL6 Antibody) at dilution of 1:1000

    Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:111748(IL6 Antibody) at dilution of 1:200 (under 10x lens)

    Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:111748(IL6 Antibody) at dilution of 1:200 (under 10x lens)

    Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:111748(IL6 Antibody) at dilution of 1:200 (under 40x lens)

    Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:111748(IL6 Antibody) at dilution of 1:200 (under 40x lens)

  • Background
    Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 Plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.
  • References
    • Shen M, Lu J, Cheng P. Ethyl pyruvate pretreatment attenuates concanavalin a-induced autoimmune hepatitis in mice. PloS one. 9(2):e87977. 2014.
    • Hu L, Li L, Xu D. Protective effects of neohesperidin dihydrochalcone against carbon tetrachloride-induced oxidative damage in vivo and in vitro. Chemico-biological interactions. 213:51-9. 2014.
    • Shen M, Lu J, Dai W. Ethyl pyruvate ameliorates hepatic ischemia-reperfusion injury by inhibiting intrinsic pathway of apoptosis and autophagy. Mediators of inflammation. 2013:461536. 2013.
    • Xia X, Su C, Fu J. Role of α-lipoic acid in LPS/d-GalN induced fulminant hepatic failure in mice: studies on oxidative stress, inflammation and apoptosis. International immunopharmacology. 22(2):293-302. 2014.
    • Xu D, Hu L, Su C. Tetrachloro-p-benzoquinone induces hepatic oxidative damage and inflammatory response, but not apoptosis in mouse: the prevention of curcumin. Toxicology and applied pharmacology. 280(2):305-13. 2014.
    • Wang C, Chen K, Xia Y. N-acetylcysteine attenuates ischemia-reperfusion-induced apoptosis and autophagy in mouse liver via regulation of the ROS/JNK/Bcl-2 pathway. PloS one. 9(9):e108855. 2014.
    • Wu X, He L, Chen F. Impaired autophagy contributes to adverse cardiac remodeling in acute myocardial infarction. PloS one. 9(11):e112891. 2014.
    • Ye Q, Zheng Y, Fan S. Lactoferrin deficiency promotes colitis-associated colorectal dysplasia in mice. PloS one. 9(7):e103298. 2014.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"