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Product Name
IL-6 antibody
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Description
IL-6 Mouse Monoclonal antibody. Positive WB detected in Jurkat cells, Recombinant protein, Transfected HEK-293 cells. Positive IHC detected in human cervical cancer tissue, human tonsillitis tissue. Observed molecular weight by Western-blot: 30 kDa
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Tested applications
ELISA, IHC, WB
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Species reactivity
Human; other species not tested.
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Alternative names
B cell stimulatory factor 2 antibody; BSF 2 antibody; BSF2 antibody; CDF antibody; CTL differentiation factor antibody; HGF antibody; HSF antibody; Hybridoma growth factor antibody; IFN beta 2 antibody; IFNB2 antibody; IL 6 antibody; IL6 antibody; Interferon beta 2 antibody; Interleukin 6 antibody
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Isotype
Mouse IgG2b
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Preparation
This antibody was obtained by immunization of IL-6 recombinant protein (Accession Number: XM_011515390). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:107402(IL6 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using Catalog No:107402(IL6 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using Catalog No:107402(IL6 Antibody) at dilution of 1:50 (under 40x lens)
Recombinant protein were subjected to SDS PAGE followed by western blot with Catalog No:107402(IL6 antibody) at dilution of 1:10000
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:107402(IL6 Antibody) at dilution of 1:1000
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Background
Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 Plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"