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Product Name
IKZF2 antibody
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Description
IKZF2 Rabbit Polyclonal antibody. Positive IP detected in Jurkat cells. Positive WB detected in human placenta tissue. Positive IHC detected in human lymphoma tissue. Observed molecular weight by Western-blot: 65 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
HELIOS antibody; IKZF2 antibody; Zinc finger protein Helios antibody; ZNF1A2 antibody; ZNFN1A2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of IKZF2 recombinant protein (Accession Number: XM_017003590). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
human placenta tissue were subjected to SDS PAGE followed by western blot with Catalog No:111648(IKZF2 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:111648(IKZF2 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:111648(IKZF2 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-IKZF2 (IP:Catalog No:111648, 4ug; Detection:Catalog No:111648 1:300) with Jurkat cells lysate 2400ug.
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Background
Ikaros DNA-binding proteins, characterized by two highly conserved zinc finger domains that mediate DNA (N-terminal domain) and protein binding (C-terminal domain), consists of 5 members and one of them is IKZF2 [PMID:1439790]. IKZF2's expression is associated with T cell activation and cellular division and is suggested a suitable marker to distinguish natural and induced Treg [PMID:21918685].
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