Cart 2

IkB alpha antibody

Cat.#: 111640

Size:

Special Price 239.0 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    IkB alpha antibody
  • Documents
  • Description
    IkB alpha Rabbit Polyclonal antibody. Positive IHC detected in human liver tissue, human cervical cancer tissue. Positive WB detected in PC-3 cells, HeLa cells, Jurkat cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 39 kDa
  • Tested applications
    ELISA, WB, IHC, IP
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    I kappa B alpha antibody; IkappaBalpha antibody; IkB alpha antibody; IKBA antibody; MAD 3 antibody; MAD3 antibody; NF kappa B inhibitor alpha antibody; NFKBI antibody; NFKBIA antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Peptide (Accession Number: NM_020529). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:500-1:5000

    IHC: 1:20-1:200

  • Validations

    PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:111640(NFKBIA antibody) at dilution of 1:500

    PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:111640(NFKBIA antibody) at dilution of 1:500

    Immunohistochemical of paraffin-embedded human liver using Catalog No:111640(NFKBIA antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human liver using Catalog No:111640(NFKBIA antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human liver using Catalog No:111640(NFKBIA antibody) at dilution of 1:100 (under 40x lens)

    Immunohistochemical of paraffin-embedded human liver using Catalog No:111640(NFKBIA antibody) at dilution of 1:100 (under 40x lens)

    IP Result of anti-NFKBIA (IP:Catalog No:111640, 4ug; Detection:Catalog No:111640 1:1000) with HeLa cells lysate 3200ug.

    IP Result of anti-NFKBIA (IP:Catalog No:111640, 4ug; Detection:Catalog No:111640 1:1000) with HeLa cells lysate 3200ug.

  • Background
    Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.
  • References
    • Zheng Z, Li H, Zhang Z. Enterovirus 71 2C protein inhibits TNF-α-mediated activation of NF-κB by suppressing IκB kinase β phosphorylation. Journal of immunology (Baltimore, Md. : 1950). 187(5):2202-12. 2011.
    • Jie L, Yong-Xiao C, Zu-Yi Y, Cang-Bao X. Minimally modified LDL upregulates endothelin type B receptors in rat coronary artery via ERK1/2 MAPK and NF-κB pathways. Biochimica et biophysica acta. 1821(4):582-9. 2012.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"