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Product Name
IkB alpha antibody
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Description
IkB alpha Rabbit Polyclonal antibody. Positive IHC detected in human liver tissue, human cervical cancer tissue. Positive WB detected in PC-3 cells, HeLa cells, Jurkat cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 39 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
I kappa B alpha antibody; IkappaBalpha antibody; IkB alpha antibody; IKBA antibody; MAD 3 antibody; MAD3 antibody; NF kappa B inhibitor alpha antibody; NFKBI antibody; NFKBIA antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_020529). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:111640(NFKBIA antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human liver using Catalog No:111640(NFKBIA antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:111640(NFKBIA antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-NFKBIA (IP:Catalog No:111640, 4ug; Detection:Catalog No:111640 1:1000) with HeLa cells lysate 3200ug.
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Background
Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.
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References
- Zheng Z, Li H, Zhang Z. Enterovirus 71 2C protein inhibits TNF-α-mediated activation of NF-κB by suppressing IκB kinase β phosphorylation. Journal of immunology (Baltimore, Md. : 1950). 187(5):2202-12. 2011.
- Jie L, Yong-Xiao C, Zu-Yi Y, Cang-Bao X. Minimally modified LDL upregulates endothelin type B receptors in rat coronary artery via ERK1/2 MAPK and NF-κB pathways. Biochimica et biophysica acta. 1821(4):582-9. 2012.
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