IGRP(206–214) peptide

IGRP(206–214) peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

H-VYLKTNVFL-OH

H-Val-Tyr-Leu-Lys-Thr-Asn-Val-Phe-Leu-OH

9

95.46%

C₅₄H₈₅N₁₁O₁₃

1096.31

Synthetic

Powder

Peptide IGRP206-214 is a specific peptide derived from the IGRP (islet-specific glucose-6-phosphatase catalytic subunit-related protein) molecule. It is a 9-amino acid peptide with the sequence VYLKTNVFL. This peptide has been studied in the context of a single-chain peptide-MHC molecule called scKd.IGRP, which contains the heavy chain of H-2Kd, b2m, and the IGRP206-214 peptide. The scKd.IGRP complex adopts a conformation in which the covalently linked IGRP206-214 peptide is properly presented for specific recognition by CD8+ T cells bearing a cognate TCR. The binding capacity of scKd.IGRP to the cognate 8.3 TCR has been demonstrated, with scKd.IGRP tetramers binding to nearly all CD8+ T cells from 8.3 TCR-transgenic NOD mice. This indicates that the scKd.IGRP complex effectively presents the IGRP206-214 peptide for recognition by the cognate TCR.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Samanta D, Mukherjee G, Ramagopal UA, Chaparro RJ, Nathenson SG, DiLorenzo TP, Almo SC. Structural and functional characterization of a single-chain peptide-MHC molecule that modulates both naive and activated CD8+ T cells. Proc Natl Acad Sci U S A. 2011 Aug 16;108(33):13682-7. doi: 10.1073/pnas.1110971108. Epub 2011 Aug 8. PMID: 21825122; PMCID: PMC3158197.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.