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Product Name
IGFBPL1 Polyclonal Antibody
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Description
Polyclonal antibody to IGFBPL1
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Tested applications
WB
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Species reactivity
Mouse, Rat
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Alternative names
IGFBPL1 antibody; IGFBP-RP4 antibody; IGFBPRP4 antibody; bA113O24.1 antibody; insulin-like growth factor-binding protein-like 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 150-250 of human IGFBPL1 (NP_001007564.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - IGFBPL1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using IGFBPL1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 300s.
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Background
IGF-binding proteins prolong the half-life of IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs in cell culture. They alter the interaction of IGFs with their cell surface receptors (By similarity). May be a putative tumor suppressor protein.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"