-
Product Name
IGF2R-Specific antibody
- Documents
-
Description
IGF2R-Specific Rabbit Polyclonal antibody. Positive FC detected in HepG2 cells. Positive IHC detected in human liver cancer tissue, human liver tissue. Positive IF detected in HepG2 cells. Positive IP detected in HepG2 cells. Positive WB detected in HepG2 cells, A431 cells, L02 cells. Observed molecular weight by Western-blot: 274 kDa
-
Tested applications
ELISA, WB, IHC, IF, IP, FC
-
Species reactivity
Human; other species not tested.
-
Alternative names
CD222 antibody; CI Man 6 P receptor antibody; CI MPR antibody; CIMPR antibody; IGF II receptor antibody; IGF2R antibody; M6P R antibody; M6P/IGF2 receptor antibody; M6P/IGF2R antibody; M6PR antibody; MPR 300 antibody; MPR1 antibody; MPRI antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_000876). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
-
Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:111682(IGF2R-Specific antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human liver cancer slide using Catalog No:111682(IGF2R-Specific Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human liver cancer slide using Catalog No:111682(IGF2R-Specific Antibody) at dilution of 1:50
IP Result of anti-IGF2R-Specific (IP:Catalog No:111682, 4ug; Detection:Catalog No:111682 1:500) with HepG2 cells lysate 3480ug.
Immunofluorescent analysis of HepG2 cells using Catalog No:111682(IGF2R-Specific Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
1X10^6 HepG2 cells were stained with .2ug IGF2R-Specific antibody (Catalog No:111682, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
-
Background
IGF2R, also named as MPRI, CI Man-6-P receptor, CI-MPR, M6PR, IGF-II receptor, M6P/IGF2R, MPR 300 and CD222, belongs to the MRL1/IGF2R family. IGF2R transports of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. IGF2R is a lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. IGF2R acts as a positive regulator of T-cell coactivation, by binding DPP4. The antibody is specific to IGF2R.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"