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IFITM2-specific antibody

Cat.#: 107276

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Product Information

  • Product Name
    IFITM2-specific antibody
  • Documents
  • Description
    IFITM2-specific Mouse Monoclonal antibody. Positive FC detected in HeLa cells. Positive WB detected in HepG2 cells, MCF7 cells, recombinant protein. Observed molecular weight by Western-blot: 15 kDa
  • Tested applications
    ELISA, WB, FC
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    DSPA2c antibody; IFITM2 antibody
  • Isotype
    Mouse IgG1
  • Preparation
    This antibody was obtained by immunization of recombinant protein (Accession Number: NM_006435). Purification method: Protein G purified.
  • Clonality
    Monoclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

  • Validations

    HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:107276(IFITM2-specific antibody) at dilution of 1:1000

    HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:107276(IFITM2-specific antibody) at dilution of 1:1000

    IFITM1 (Lane 1), IFITM2 (Lane 2) and IFITM3 (Lane 3) fusion proteins (20ng/lane) were subjected to SDS-PAGE followed by western blot with IFITM2 monoclonal antibody (Catalog No:107276) at dilution of 1:10,000.

    IFITM1 (Lane 1), IFITM2 (Lane 2) and IFITM3 (Lane 3) fusion proteins (20ng/lane) were subjected to SDS-PAGE followed by western blot with IFITM2 monoclonal antibody (Catalog No:107276) at dilution of 1:10,000.

    1X10^6 HeLa cells were stained with 0.2ug IFITM2-specific antibody (Catalog No:107276, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

    1X10^6 HeLa cells were stained with 0.2ug IFITM2-specific antibody (Catalog No:107276, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

  • Background
    IFITM2, also named as 1-8D, belongs to the CD225 family. It is an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus (WNV), by inhibiting the early step(s) of replication. IFITM2 induces cell cycle arrest and mediates apoptosis by caspase activation and in p53-independent manner. It is overexpressed in colon carcinoma. IFITM2 is a novel pro-apoptotic gene that will provide new insights into the regulated cellular pathways to death.(PMID:19544527) This antibody is specific to IFTIM2.
  • References
    • Zhu X, He Z, Yuan J. IFITM3-containing exosome as a novel mediator for anti-viral response in dengue virus infection. Cellular microbiology. 17(1):105-18. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"