-
Product Name
IFIH1 antibody
- Documents
-
Description
IFIH1 Rabbit Polyclonal antibody. Positive IP detected in Jurkat cells. Positive WB detected in Jurkat cells. Observed molecular weight by Western-blot: 117-125kd
-
Tested applications
ELISA, WB, IP
-
Species reactivity
Human; other species not tested.
-
Alternative names
Helicard antibody; Helicase with 2 CARD domains antibody; Hlcd antibody; IDDM19 antibody; IFIH1 antibody; MDA 5 antibody; MDA5 antibody; RH116 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of IFIH1 recombinant protein (Accession Number: NM_022168). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
-
Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:111616(IFIH1 antibody) at dilution of 1:1500
IP Result of anti-IFIH1 (IP:Catalog No:111616, 4ug; Detection:Catalog No:111616 1:800) with Jurkat cells lysate 2400ug.
-
Background
IFIH1 (Interferon-induced helicase C domain-containing protein 1) is a putative RNA helicase that is upregulated in response to treatment with IFNB or IFNB and MEZ. It is also named as MDA5 and RH116. Ectopic expression of MDA5 in melanoma cells resulted in reduced colony formation, suggesting an interaction of the CARD and apoptotic signal molecules. Functional analysis indicated that MDA5 is an RNA-dependent ATPase.This antibody is specific to IFIH1.
-
References
- Zhang W, Zhang L, Zan Y, Du N, Yang Y, Tien P. Human respiratory syncytial virus infection is inhibited by IFN-induced transmembrane proteins. The Journal of general virology. 96(Pt 1):170-82. 2015.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"