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Product Name
IDH3A antibody
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Description
IDH3A Rabbit Polyclonal antibody. Positive IP detected in HepG2 cells. Positive WB detected in human skeletal muscle tissue, HepG2 cells, mouse brain tissue. Positive IHC detected in human brain tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 40 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
IDH3A antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of IDH3A recombinant protein (Accession Number: NM_005530). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with Catalog No:111605(IDH3A antibody) at dilution of 1:1200
Immunohistochemical of paraffin-embedded human brain using Catalog No:111605(IDH3A antibody) at dilution of 1:100 (under 40x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:111605(IDH3A antibody) at dilution of 1:100 (under 10x lens)
IP Result of anti-IDH3A (IP:Catalog No:111605, 4ug; Detection:Catalog No:111605 1:1000) with HepG2 cells lysate 2400ug.
Immunofluorescent analysis of HepG2 cells using Catalog No:111605(IDH3A Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
IDH3A represents isocitrate dehydrogenase 3 (NAD+) alpha
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References
- Völgyi K, Gulyássy P, Háden K. Synaptic mitochondria: a brain mitochondria cluster with a specific proteome. Journal of proteomics. 120:142-57. 2015.
- Oda T, Yamaguchi A, Shimizu K, Nikai T, Matsumoto K. Does the Rewarmed Heart Restore the Myocardial Proteome to That of the Pre-Cooled State?--A Proteomic Analysis of Surgical Samples. Circulation journal : official journal of the Japanese Circulation Society. 79(12):2648-58. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"