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Product Name
IDH1 Polyclonal Antibody
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Description
Polyclonal antibody to IDH1
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Tested applications
WB, IF, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
IDH1 antibody; HEL-216 antibody; HEL-S-26 antibody; IDCD antibody; IDH antibody; IDP antibody; IDPC antibody; PICD antibody; isocitrate dehydrogenase [NADP] cytoplasmic antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-414 of human IDH1 (NP_005887.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200
IP 1:50 - 1:100 -
Validations
Western blot - IDH1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using IDH1 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunoprecipitation - IDH1 Polyclonal Antibody
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug IDH1 antibody . Western blot was performed from the immunoprecipitate using IDH1 antibody at a dilition of 1:1000.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"