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Product Name
IDE Polyclonal Antibody
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Description
Polyclonal antibody to IDE
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Tested applications
WB, IF
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Species reactivity
Human, Mouse
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Alternative names
IDE antibody; INSULYSIN antibody; insulin-degrading enzyme antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human IDE (NP_004960.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:10 - 1:100 -
Validations
Western blot - IDE Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using IDE antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .
Western blot - IDE Polyclonal Antibody
Western blot analysis of extracts from normal (control) and IDE knockout HeLa cells, using IDE antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
Immunofluorescence - IDE Polyclonal Antibody
Immunofluorescence analysis of A549 cells using IDE antibody . Blue: DAPI for nuclear staining.
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Background
Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia.; (Microbial infection) The membrane-associated isoform acts as an entry receptor for varicella-zoster virus (VZV).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"