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Product Name
IDE antibody
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Description
IDE Rabbit Polyclonal antibody. Positive IP detected in HepG2 cells. Positive WB detected in HepG2 cells, A2780 cells, DU 145 cells, mouse liver tissue, SH-SY5Y cells. Positive IHC detected in human kidney tissue, human liver tissue. Observed molecular weight by Western-blot: 100-120 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Abeta degrading protease antibody; FLJ35968 antibody; IDE antibody; insulin degrading enzyme antibody; Insulin protease antibody; Insulinase antibody; INSULYSIN antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of IDE recombinant protein (Accession Number: NM_004969). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:111600(IDE antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human kidney using Catalog No:111600(IDE antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:111600(IDE antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-IDE (IP:Catalog No:111600, 4ug; Detection:Catalog No:111600 1:500) with HepG2 cells lysate 1600ug.
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Background
IDE(Insulin-degrading enzyme) belongs to the peptidase M16 family. It is also known as insulysin and is a 110-kD neutral metallopeptidase that can degrade a number of peptides, including insulin and beta-amyloid(PMID:9830016). The reduction of IDE expression may be a risk factor for AD, and that IDE may interact with APOE status to affect beta-amyloid metabolism(PMID:12507914). It can exsit as a homodimer and form higher oligomers(PMID:17613531). This antibody is specific to IDE.
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References
- Yang W, Ma J, Liu Z, Lu Y, Hu B, Yu H. Effect of naringenin on brain insulin signaling and cognitive functions in ICV-STZ induced dementia model of rats. Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology. 35(5):741-51. 2014.
- Kakiya N, Saito T, Nilsson P. Cell surface expression of the major amyloid-β peptide (Aβ)-degrading enzyme, neprilysin, depends on phosphorylation by mitogen-activated protein kinase/extracellular signal-regulated kinase kinase (MEK) and dephosphorylation by protein phosphatase 1a. The Journal of biological chemistry. 287(35):29362-72. 2012.
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