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Product Name
ID3 Polyclonal Antibody
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Description
Polyclonal antibody to ID3
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
ID3 antibody; HEIR-1 antibody; bHLHb25 antibody; DNA-binding protein inhibitor ID-3 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-119 of human ID3 (NP_002158.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100 -
Validations
Western blot - ID3 Polyclonal Antibody
Western blot analysis of extracts of mouse liver, using ID3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry - ID3 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using ID3 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - ID3 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse kidney using ID3 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - ID3 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse pancreas using ID3 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - ID3 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using ID3 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - ID3 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse spleen using ID3 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - ID3 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human lung cancer using ID3 antibody at dilution of 1:200 (40x lens).
Immunofluorescence - ID3 Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using ID3 antibody .
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Background
Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Involved in myogenesis by inhibiting skeletal muscle and cardiac myocyte differentiation and promoting muscle precursor cells proliferation. Inhibits the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer.
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