-
Product Name
HYLS1 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to HYLS1
-
Tested applications
WB, IF
-
Species reactivity
Human
-
Alternative names
HYLS1 antibody; HLS antibody; hydrolethalus syndrome protein 1 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 70-299 of human HYLS1 (NP_659451.1).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - HYLS1 Polyclonal Antibody
Western blot analysis of extracts of MCF-7 cells, using HYLS1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - HYLS1 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using HYLS1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
-
Background
Plays a role in ciliogenesis.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"