HYI Polyclonal Antibody

Cat.#: 161267

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Product Information

  • Product Name
    HYI Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to HYI
  • Tested applications
    WB
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    HYI antibody; HT036 antibody; putative hydroxypyruvate isomerase antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 80-260 of human HYI (NP_001177809.1).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
  • Validations

    Western blot - HYI Polyclonal Antibody

    Western blot - HYI Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using HYI antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.

  • Background
    Catalyzes the reversible isomerization between hydroxypyruvate and 2-hydroxy-3-oxopropanoate (also termed tartronate semialdehyde).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"