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Product Name
HVCN1 antibody
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Description
HVCN1 Rabbit Polyclonal antibody. Positive IHC detected in human tonsillitis tissue. Positive IF detected in PC-3 cells. Positive WB detected in Raji cells, PC-3 cells. Observed molecular weight by Western-blot: 28-32 kDa; ~60 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human; other species not tested.
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Alternative names
HV1 antibody; HVCN1 antibody; VSOP antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HVCN1 recombinant protein (Accession Number: XM_011538845). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
Raji cells were subjected to SDS PAGE followed by western blot with Catalog No:111730(HVCN1 Antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:111730(HVCN1 Antibody) at dilution of 1:200 (under 10x lens)
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:111730(HVCN1 Antibody) at dilution of 1:200 (under 40x lens)
Immunofluorescent analysis of (-20oc Ethanol) fixed PC-3 cells using Catalog No:111730(HVCN1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
HVCN1, also named as VSOP and HV1, Belongs to the hydrogen channel family. HVCN1 mediates the voltage-dependent proton permeability of excitable membranes. It forms a proton-selective channel through which protons may pass in accordance with their electrochemical gradient. Proton efflux, HVCN1 is accompanied by membrane depolarization, facilitates acute production of reactive oxygen species in phagocytosis. HVCN1, the voltage-sensitive proton channel, is present in human sperm and is an important regulator of the functional maturation of sperm. HVCN1 has four isoforms with MW 28-32 kDa or 40 kDa (modification). It has a dimer form with MW ~60 kDa.
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