HUS1 Polyclonal Antibody

Cat.#: 161858

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Product Information

  • Product Name
    HUS1 Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to HUS1
  • Tested applications
    WB, IHC, IF
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    HUS1 antibody; hHUS1 antibody; checkpoint protein HUS1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human HUS1 (NP_004498.1).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IHC 1:50 - 1:100
    IF 1:50 - 1:100
  • Validations

    Western blot - HUS1 Polyclonal Antibody

    Western blot - HUS1 Polyclonal Antibody

    Western blot analysis of extracts of mouse kidney, using HUS1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 180s.

    Immunohistochemistry - HUS1 Polyclonal Antibody

    Immunohistochemistry - HUS1 Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded rat liver using HUS1 antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - HUS1 Polyclonal Antibody

    Immunohistochemistry - HUS1 Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded rat heart using HUS1 antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - HUS1 Polyclonal Antibody

    Immunohistochemistry - HUS1 Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded human gastric cancer using HUS1 antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - HUS1 Polyclonal Antibody

    Immunohistochemistry - HUS1 Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded human uterine cancer using HUS1 antibody at dilution of 1:100 (40x lens).

  • Background
    Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"