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Product Name
HuR antibody
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Description
HuR Rabbit Polyclonal antibody. Positive FC detected in MCF-7 cells. Positive IHC detected in human ovary tumor tissue, human breast cancer tissue, human pancreas tissue, human skin tissue. Positive IF detected in MCF-7 cells, Ethacrynic acid treated HepG2 cells. Positive IP detected in HEK-293 cells. Positive WB detected in HEK-293 cells, A431 cells, HeLa cells, hESC cells, HL-60 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, RAW264.7 cells. Observed molecular weight by Western-blot: 30-37kd
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Tested applications
ELISA, WB, IF, IHC, FC, IP
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Species reactivity
Human,Mouse,Rat,Zebrafish; other species not tested.
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Alternative names
ELAV like protein 1 antibody; ELAV1 antibody; ELAVL1 antibody; Hu antigen R antibody; Hua antibody; HuR antibody; MelG antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HuR recombinant protein (Accession Number: NM_001419). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:111725(HuR antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human ovary tumor slide using Catalog No:111725(HuR Antibody) at dilution of 1:50
Immunofluorescent analysis of MCF-7 cells, using ELAVL1 antibody Catalog No:111725 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-HuR (IP:Catalog No:111725, 4ug; Detection:Catalog No:111725 1:1000) with HEK-293 cells lysate 2800ug.
1X10^6 MCF-7 cells were stained with 0.2ug HuR antibody (Catalog No:111725, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
ELAVL1, also named as HUR, belongs to the RRM elav family. It is involved in 3'-UTR ARE-mediated MYC stabilization. ELAVL1 binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, ELAVL1 binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.
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References
- Luo NA, Qu YQ, Yang GD. Post-transcriptional up-regulation of PDGF-C by HuR in advanced and stressed breast cancer. International journal of molecular sciences. 15(11):20306-20. 2014.
- Wu S, Wang Y, Lin L. Protease 2A induces stress granule formation during coxsackievirus B3 and enterovirus 71 infections. Virology journal. 11:192. 2014.
- Wang Y, Yue D, Xiao M. C1QBP negatively regulates the activation of oncoprotein YBX1 in the renal cell carcinoma as revealed by interactomics analysis. Journal of proteome research. 14(2):804-13. 2015.
- Li H, Hou S, Hao T. HuR antagonizes the effect of an intronic pyrimidine-rich sequence in regulating WT1 +/-KTS isoforms. RNA biology. 12(12):1364-71. 2015.
- Kuś K, Rakus K, Boutier M. The Structure of the Cyprinid herpesvirus 3 ORF112-Zα·Z-DNA Complex Reveals a Mechanism of Nucleic Acids Recognition Conserved with E3L, a Poxvirus Inhibitor of Interferon Response. The Journal of biological chemistry. 290(52):30713-25. 2015.
- Zhou J, Yang L, Zhong T. H19 lncRNA alters DNA methylation genome wide by regulating S-adenosylhomocysteine hydrolase. Nature communications. 6:10221. 2015.
- Zhao W, Zhao J, Hou M. HuR and TIA1/TIAL1 are involved in regulation of alternative splicing of SIRT1 pre-mRNA. International journal of molecular sciences. 15(2):2946-58. 2014.
- Wu S, Lin L, Zhao W. AUF1 is recruited to the stress granules induced by coxsackievirus B3. Virus research. 192:52-61. 2014.
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