-
Product Name
Human TrkB/NTRK2 (His & Fc Tag) recombinant protein
- Documents
-
Description
TrkB receptor also known as TrkB tyrosine kinase or BDNF/NT-3 growth factors receptor or neurotrophic tyrosine kinase, receptor, type 2 (NTRK2) is a single transmembrane catalytic receptors with intracellular tyrosine kinase activity. TrkB/NTRK2 is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. TrkB tyrosine kinase (TrkB) or NTRK2 is coupled to the Ras, Cdc42/Rac/RhoG, MAPK, PI3-K and PLCgamma signaling pathways. There are four members of the Trk family; TrkA, TrkB and TrkC and a related p75NTR receptor. Each family member binds different neurotrophins with varying affinities. TrkB/NTRK has highest affinity for brain-derived neurotrophic factor (BDNF) and is involved in neuronal plasticity, longterm potentiation and apoptosis of CNS neurons. Other neurotrophins include nerve growth factor(NGF), neurotrophin-3 and neurotrophin-4. TrkB/NTRK is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in TrkB/NTRK have been associated with obesity and mood disorders.
-
Protein name
BDNF/NT-3 growth factors receptor
-
Protein short names
GP145-TRKB; GP145-TRKB/GP95-TRKB; TRKB; TRK-B; NTRK2; C030027L06RIK; TKRB; AI848316
-
Uniprot ID
Q5VWE5
-
Gene Name
NTRK2; TRKB
-
Source/Expression Host
Human Cells
-
Expression Plasmid/cDNA
A DNA sequence encoding the extracellular domain (Met 1-His 430) of human TrkB (NP_001007098.1) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the carboxy-terminus.
-
Protein Species
Human
-
Molecular weight
The recombinant human TrkB/Fc is a disulfide-linked homodimer. The reduced monomer consists of 646 amino acids and has a predicted molecular mass of 72 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhTrkB/Fc monomer is approximately 110-120 kDa due to glycosylation.
-
Purity
> 90 % as determined by SDS-PAGE
-
Activity
Measured by its ability to bind mouse BDNF in functional ELISA.
-
Validations
Human TrkB / NTRK2 Protein (His & Fc Tag) SDS-PAGE
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"