-
Product Name
Human TGFBR1 / ALK-5 (aa 200-503, His & GST Tag) recombinant protein
- Documents
-
Description
Transforming growth factor, beta receptor I, also known as Transforming growth factor-beta receptor type I , Serine / threonine-protein kinase receptor R4, Activin receptor-like kinase 5, SKR4, ALK-5, and TGFBR1, is a single-pass type I membrane protein which belongs to the protein kinase superfamily and TGFB receptor subfamily. TGFBR1 / ALK-5 is found in all tissues examined. It is most abundant in placenta and least abundant in brain and heart. TGF-beta functions as a tumor suppressor by inhibiting the cell cycle in the G1 phase. Administration of TGF-beta is able to protect against mammary tumor development in transgenic mouse models in vivo. Disruption of the TGF-beta/SMAD pathway has been implicated in a variety of human cancers, with the majority of colon and gastric cancers being caused by an inactivating mutation of TGF-beta RII. On ligand binding, TGFBR1 / ALK-5 forms a receptor complex consisting of two type I I and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which auto-phosphorylate, then bind and activate SMAD transcriptional regulators. TGF-beta signaling via TGFBR1 / ALK-5 is not required in myocardial cells during mammalian cardiac development, but plays an irreplaceable cell-autonomous role regulating cellular communication, differentiation and proliferation in endocardial and epicardial cells. Defects in TGFBR1 / ALK-5 are the cause of Loeys-Dietz syndrome type 1A (LDS1A), Loeys-Dietz syndrome type 2A (LDS2A), and aortic aneurysm familial thoracic type 5 (AAT5).
-
Protein name
TGF-beta receptor type-1
-
Protein short names
AAT5; ESK2; LDS2A; TBETAR-I; ALK5; ALK-5; ESS1; RP23-457P12.1; TGFR-1; ACVRLK4; MSSE; TGFBR1; TBETARI; AU017191; LDS1; SKR4; LDS1A
-
Uniprot ID
P36897
-
Gene Name
TGFBR1; ALK5; SKR4
-
Source/Expression Host
Baculovirus-Insect Cells
-
Expression Plasmid/cDNA
A DNA sequence encoding the human ALK5 (P36897-1) (Thr200-Mey503) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
-
Protein Species
Human
-
Molecular weight
The recombinant human ALK5/GST chimera consists of 541 amino acids and has a calculated molecular mass of 62.6 kDa. The recombinant protein migrates approximately 57 kDa band in SDS-PAGE under reducing conditions.
-
Purity
> 95 % as determined by SDS-PAGE
-
Activity
The specific activity was determined to be 40 nmol/min/mg using casein as substrate.
-
Validations
Human TGFBR1 / ALK-5 / SKR4 Protein (aa 200-503, His & GST Tag) SDS-PAGE
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"