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Product Name
Human SLPI (His Tag) recombinant protein
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Description
Secretory leukoprotease inhibitor (SLPI), also called antileukoprotease (ALP), is a 12-kDa, nonglycosylated serine protease inhibitor present in mucous secretions. It is thought to play a role in protecting the mucosae from injury associated with inflammation. SLPI is locally produced by serous cells, including bronchial submucosal glands. Elafin and SLPI are members of larger families of proteins secreted predominantly at mucosal sites, and have been shown to be modulated in multiple pathological conditions. Elafin and SLPI are structurally related in that both have a fold with a four-disulfide core or whey acidic protein (WAP) domain responsible for inhibiting proteases. SLPI is a prominent innate immune protein of the respiratory tract, possessing serine protease inhibitor activity, antibacterial activity, and anti-inflammatory/immunomodulatory activity.
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Protein name
Antileukoproteinase
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Protein short names
WFDC4; BLPI; ALK1; MPI; RP23-462O16.7; WAP4; SLPI; HUSI; HUSI-I; ALP
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Uniprot ID
P03973
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Gene Name
SLPI; WAP4; WFDC4
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Source/Expression Host
Baculovirus-Insect Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the human SLPI (P03973)( Met1-Ala132) was expressed, with a C-terminal polyhistidine tag.
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Protein Species
Human
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Molecular weight
The secreted recombinant human SLPI consists of 117 amino acids and predicts a molecular mass of 13.1 KDa. The apparent molecular mass of the protein is approximately 15 KDa in SDS-PAGE under reducing conditions due to glycosylation.
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Purity
> 90 % as determined by SDS-PAGE
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Activity
Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK (Dnp)-NH2 (Catalog # ES002).
The IC50 value is <1 Nm. -
Validations
Human SLPI Protein (His Tag) SDS-PAGE
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