Human PTK2B Recombinant protein (His tag)

Human PTK2B Recombinant protein (His tag)

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Non-receptor protein-tyrosine kinase that regulates reorganization of the actin cytoskeleton, cell polarization, cell migration, adhesion, spreading and bone remodeling. Plays a role in the regulation of the humoral immune response, and is required for normal levels of marginal B-cells in the spleen and normal migration of splenic B-cells. Required for normal macrophage polarization and migration towards sites of inflammation. Regulates cytoskeleton rearrangement and cell spreading in T-cells, and contributes to the regulation of T-cell responses. Promotes osteoclastic bone resorption; this requires both PTK2B/PYK2 and SRC. May inhibit differentiation and activity of osteoprogenitor cells. Functions in signaling downstream of integrin and collagen receptors, immune receptors, G-protein coupled receptors (GPCR), cytokine, chemokine and growth factor receptors, and mediates responses to cellular stress. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade. Promotes activation of NOS3. Regulates production of the cellular messenger cGMP. Promotes activation of the MAP kinase signaling cascade, including activation of MAPK1/ERK2, MAPK3/ERK1 and MAPK8/JNK1. Promotes activation of Rho family GTPases, such as RHOA and RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Acts as a scaffold, binding to both PDPK1 and SRC, thereby allowing SRC to phosphorylate PDPK1 at 'Tyr-9, 'Tyr-373', and 'Tyr-376'. Promotes phosphorylation of NMDA receptors by SRC family members, and thereby contributes to the regulation of NMDA receptor ion channel activity and intracellular Ca(2+) levels. May also regulate potassium ion transport by phosphorylation of potassium channel subunits. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ASAP1, NPHP1, KCNA2 and SHC1. Promotes phosphorylation of ASAP2, RHOU and PXN; this requires both SRC and PTK2/PYK2. {PubMed:10022920, PubMed:12771146, PubMed:12893833, PubMed:14585963, PubMed:15050747, PubMed:15166227, PubMed:17634955, PubMed:18086875, PubMed:18339875, PubMed:18587400, PubMed:18765415, PubMed:19086031, PubMed:19207108, PubMed:19244237, PubMed:19428251, PubMed:19648005, PubMed:19880522, PubMed:20001213, PubMed:20381867, PubMed:20521079, PubMed:21357692, PubMed:21533080, PubMed:7544443, PubMed:8670418, PubMed:8849729}.

Protein-tyrosine kinase 2-beta

FAK2; PYK2; RAFTK

Q14289

PTK2B; FAK2; PYK2; RAFTK

E. coli

A DNA sequence encoding the human PTK2B (XP_047277494.1) 646-1009 aa was fused with the polyhistidine tag

Human

Not tested.