Human PGA4/Pepsinogen A (Fc Tag) recombinant protein

Human PGA4/Pepsinogen A (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

PGA4 (Pepsinogen 4, group I), or Pepsinogen A, is a member of the peptidase A1 family. Pepsin is expressed as a pro-form zymogen, pepsinogen, whose primary structure has an additional 44 amino acids. Pepsin is stored as pepsinogen so it will only be released when needed, and does not digest the body's own proteins in the stomach's lining. Five types of zymogens of pepsins, gastric digestive proteinases, are known: pepsinogens A, B, and F, progastricsin, and prochymosin. There are two major groups of pepsinogen, namely pepsinogen A (PGA) and pepsinogen C (PGC) (or progastricsin), and each frequently has isozymogens. The PGA3, PGA4 and PGA5 genes encode identical human pepsinogen A enzymes.

PGA4; PGA3; PEPSINOGEN A; PGA5; FLJ58952; FLJ77962

P0DJD9

Human Cells

A DNA sequence encoding the human PGA4 (P00790) (Met 1-Ala 388) was fused with the Fc region of human IgG1 at the C-terminus.

Human

The secreted recombinant human PGA4/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 614 amino acids and has a predicted molecular mass of 67.3 kDa. The apparent molecular mass of rhPGA/Fc monomer is approximately 65-70 kDa in SDS-PAGE under reducing conditions.

> 80 % as determined by SDS-PAGE

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2, AnaSpec, Catalog # 27114.
The specific activity is > 3000 pmoles/min/μg.