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Product Name
Anti-PGA4/Pepsinogen A antibody
- Documents
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Description
Rabbit polyclonal to PGA4/Pepsinogen A
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Tested applications
ELISA, IHC-P
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Species reactivity
Human PGA4 / Pepsinogen A
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Alternative names
Pepsinogen A antibody; PGA4 antibody; PGA5 antibody; FLJ58952 antibody; FLJ77962 antibody; PGA3 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human PGA4 / Pepsinogen A (rh PGA4 / Pepsinogen A; P00790; Met1-Ala388). PGA4 / Pepsinogen A specific IgG was purified by Human PGA4 / Pepsinogen A affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.1-0.2 μg/ml
This antibody can be used at 0.1-0.2 μg/ml with the appropriate secondary reagents to detect Human PGA4 / Pepsinogen A. The detection limit for Human PGA4 / Pepsinogen A is approximately < 0.039 ng/well.
IHC-P: 0.1-2 μg/ml
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Validations
PGA4 / Pepsinogen A Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human PGA in human gastric cancer with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections).
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Background
PGA4 (Pepsinogen 4, group I), or Pepsinogen A, is a member of the peptidase A1 family. Pepsin is expressed as a pro-form zymogen, pepsinogen, whose primary structure has an additional 44 amino acids. Pepsin is stored as pepsinogen so it will only be released when needed, and does not digest the body's own proteins in the stomach's lining. Five types of zymogens of pepsins, gastric digestive proteinases, are known: pepsinogens A, B, and F, progastricsin, and prochymosin. There are two major groups of pepsinogen, namely pepsinogen A (PGA) and pepsinogen C (PGC) (or progastricsin), and each frequently has isozymogens. The PGA3, PGA4 and PGA5 genes encode identical human pepsinogen A enzymes.
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References
- Kageyama T. (2002) Pepsinogens, progastricsins, and prochymosins: structure, function, evolution, and development. Cell Mol Life Sci. 59(2): 288-306.
- Takahashi K. (1992) Gene structures of pepsinogens A and C. Scand J Clin Lab Invest Suppl. 210: 97-110.
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