Human MICB (His & Fc Tag) recombinant protein

Human MICB (His & Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

MHC class I polypeptide-related sequence B, also known as MICB, is a heavily glycosylated protein serving as a ligand for the type I I receptor NKG2D. MICB shares 85% amino acid identity with MICA, a closely related protein, both of which contain three extracellular immunoglobulin-like domains, but without capacity to bind peptide or interact with beta-2-microglobulin. acting as a stress-induced self-antigen, binding of MICB to the NKG2D receptor activates the cytolytic response of natural killer (NK) cells, CD8+?? T cells, and ?? T cells on which the receptor is expressed. MICA/B are minimally expressed on normal cells, but are frequently expressed on epithelial tumors and can be induced by bacterial and viral infections. MICA/B recognition thus is involved in tumor surveillance, viral infections, and autoimmune diseases.

MHC class I polypeptide-related sequence B

PERB11.2; DAAP-210H10.1; MICB

X6R344

MICB; PERB11.2

Human Cells

A DNA sequence encoding the extracellular domain of human MICB (NP_005922.2) (Met 1-Gly 298) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Human

The recombinant human MICB/Fc is a disulfide-linked homodimer. The reduced monomer consists of 524 amino acids and has a predicted molecular mass of 59.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh MICB/Fc monomer is approximately 80-90 kDa due to glycosylation.

> 98 % as determined by SDS-PAGE

Immobilized human His-NKG2D (78-216) (Cat:504733) at 10 μg/ml (100 μl/well) can bind human MICB-Fch, The EC₅₀ of human MICB-Fch is 15.9-37.1 ng/ml.