Human KIRREL1/NEPH1 (Fc Tag) recombinant protein

Human KIRREL1/NEPH1 (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

NEPH1 (KIRREL1) belongs to a family of three closely related transmembrane proteins of the Ig superfamily with a structure similar to that of nephrin. All three Neph proteins share a conserved podocin-binding motif; mutation of a centrally located tyrosine residue dramatically lowers the affinity of Neph1 for podocin. Neph1 triggers AP-1 activation similarly to nephrin but requires the presence of Tec family kinases for efficient transactivation. Neph1 consists of a signal peptide, five Ig-like C2-type domains with the middle domain overlapping with a PKD-like domain, an RGD sequence, a transmembrane domain and a cytoplasmic tail, which is expressed in slit diaphragm domains of podocytes and in vertebrate and invertebrate nervous systems. Neph1 is abundantly expressed in the kidney, specifically expressed in podocytes of kidney glomeruli, and plays a significant role in the normal development and function of the glomerular permeability. Neph1 interacts with nephrin in vitro and in vivo, and able to stimulate transcriptional activation in a model system, such as the activation the transcription factor AP-1 via the stimulation of a MAPK module. Neph1 is crucial for the integrity of the slit diaphragm, as Neph1 gene knockout mice results in effacement of glomerular podocytes, heavy proteinuria, and early postnatal death.

Kin of IRRE-like protein 1

Q96J84

KIRREL; KIRREL1; NEPH1

Human Cells

A DNA sequence encoding the human KIRREL (NP_060710.3) (Met1-Leu493) was expressed with the Fc region of human IgG1 at the C-terminus.

Human

The recombinant human KIRREL/Fc comprises 715 amino acids and has a predicted molecular mass of 78.8 kDa. The apparent molecular mass of the protein is approximately 96.1 kDa in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE

Measured by the ability of the immobilized protein to support the adhesion of MS1 mouse pancreatic islet endothelial cells.
When cells are added to KIRREL coated plates (15 μg/mL, 100 μL/well), > 20% will adhere specifically after 90 minutes at 37 ℃.