Human JAG1/Jagged 1(CD339) (His Tag) recombinant protein

Human JAG1/Jagged 1(CD339) (His Tag) recombinant protein

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Protein Jagged 1, also known as JAG1, JAGL1 and CD339, is a single-pass type I  membrane protein which contains 1 DSL domain and 15 EGF-like domains. JAG1/Jagged 1 is widely expressed in adult and fetal tissues. The expression of JAG1/Jagged 1 is up-regulated in cervical squamous cell carcinoma. JAG1/Jagged 1 is also expressed in bone marrow cell line HS-27a which supports the long-term maintenance of immature progenitor cells. JAG1/Jagged 1 is a ligand for multiple Notch receptors. It is involved in the mediation of Notch signaling. JAG1/Jagged 1 may be involved in cell-fate decisions during hematopoiesis.  JAG1/Jagged 1 seems to be involved in early and late stages of mammalian cardiovascular development. It inhibits myoblast differentiation and enhances fibroblast growth factor-induced angiogenesis. Defects in JAG1/Jagged 1 are the cause of Alagille syndrome type 1 (ALGS1). Alagille syndrome is an autosomal dominant multisystem disorder defined clinically by hepatic bile duct paucity and cholestasis in association with cardiac, skeletal, and ophthalmologic manifestations. Defects in JAG1/Jagged 1 are also a cause of tetralogy of Fallot (TOF). TOF is a congenital heart anomaly which consists of pulmonary stenosis, ventricular septal defect, dextroposition of the aorta (aorta is on the right side instead of the left) and hypertrophy of the right ventricle. This condition results in a blue baby at birth due to inadequate oxygenation.

Protein jagged-1

SERRATE-1; HJ1; JAG1; RP23-173O12.3; GSFABE2; JAGGED 1; MGC104644; HTU; SER-1; JAGL1; HEADTURNER; SLALOM; OZZ; ABE2; AHD; AWS; AGS; CD339

Q99740

JAG1; JAGL1

Human Cells

A DNA sequence encoding the human JAG1 (NP_000205.1) extracellular domain (Met 1-Ser 1046) was expressed, fused with a polyhistidine tag at the C-terminus.

Human

The recombinant human JAG1 consists of 1024 amino acids and has a predicted molecular mass of 112 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh JAG1 is approximately 160-180 kDa due to glycosylation.

> 85 % as determined by SDS-PAGE

Measured by the ability of the immobilized protein to enhance BMP2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells.
The ED₅₀ for this effect is typically 4-20 μg/mL.